Description
The human IL1R2 (IL1RB / CD121b) ELISA Pair Set is for the quantitative determinationof human IL1R2. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL1R2(IL1RB / CD121b) coated on a 96-well plate. Standards andsamples are added to the wells, and any IL1R2 ( IL1RB / CD121b) present binds tothe immobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-IL1R2 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof IL1R2 present in the sample. To end the enzyme reaction, the stop solutionis added and absorbances of the microwell are read at 450 nm